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Antibodies

HIV1 p24 Recombinant Mouse monoclonal Antibody

Product Sizes
100μg
£689.00
HA601104-100UG
About this Product
SKU:
HA601104
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
12G4-R
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278).
Isotype:
IgG1
Molecular Weight:
Predicted band size: 24 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Standard curve of p24 matched pair antibodies: Sandwich ELISA analysis of p24 matched piar antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA601104 [12G4-R] diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150ul 1% BSA/PBST blocking buffer; and incubated with serial diluted recombinant p24 protein starting from 1000pg/ml to 15.6pg/ml for 1 hours at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA601105 [12G2-R] (Biotin; 1:2;000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies; recombinant p24 protein; Streptavidin-HRP are diluted in 1% BSA/PBST.