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Antibodies

DYKDDDDK Tag (FLAG) Recombinant Mouse monoclonal Antibody

Product Sizes
100μl
£434.00
HA600138F-100UL
About this Product
SKU:
HA600138F
Buffer:
Supplied in phosphate-buffered solution; pH 7.2; containing 0.2% ProClean 950 and BSA.
translate.label.attr.clone:
A2-A4-R
Clonality:
Monoclonal
Conjugate:
iFluor™ 594
Host:
Mouse
Immunogen:
Synthetic peptide immune sequence is N-DYKDDDDK-C.
Isotype:
IgG1
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of HeLa cells labeling DYKDDDDK Tag (FLAG) with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA600138F) at 1/500 dilution. HeLa cells; transfected with empty control (top; negative) / Flag-tagged Claudin 18.2 (middle; positive) / Flag-tagged Histone H3.1 (bottom; positive) expression vector; respectively; were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA600138F; iFluor™ 594; red) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.