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Antibodies

AMPK alpha 2 Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
HA600079-100UL
About this Product
SKU:
HA600079
Application:
IHC-Paraffin
Buffer:
1*TBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A6A11
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human AMPK alpha 2 aa 231-430.
Isotype:
IgG1
Molecular Weight:
62 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
ICC staining of AMPK alpha 2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600079; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA600079; 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA600079; 1/1;000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AMPK alpha 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA600079; 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.