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Antibodies

Histone H3 (acetyl K27) Mouse monoclonal Antibody

Product Sizes
50uL
£245.00
HA600047-50UL
100μl
£400.00
HA600047-100UL
About this Product
SKU:
HA600047
Application:
Western Blot, IHC-Paraffin, Flow Cytometry, ChIP
Buffer:
PBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A6D7
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within human Histone H3 aa 20-40 (acetyl K27).
Isotype:
IgG1
Molecular Weight:
Predicted band size: 15 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Histone H3 (acetyl K27) on different lysates with Mouse anti-Histone H3 (acetyl K27) antibody (HA600047) at 1/1;000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 1μM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600047) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 (acetyl K27) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600047; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20;000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate
Western blot analysis of Histone H3 (acetyl K27) on HeLa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600047; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20;000 dilution was used for 1 hour at room temperature. Lane 1: Untreated HeLa whole cell lysates Lane 2: HeLa treated with Trichostatin A whole cell lysates
Immunocytochemistry analysis of HUVEC cells labeling Histone H3 (acetyl K27) with Mouse anti-Histone H3 (acetyl K27) antibody (HA600047) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K27) antibody (HA600047) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) was used as the secondary antibody at 1/1;000 dilution.