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Antibodies

TROP2 Recombinant Chimeric Antibody Antibody

Product Sizes
100μl
£370.00
HA600012-100UL
About this Product
SKU:
HA600012
Application:
ELISA, Flow Cytometry
Buffer:
1*PBS (pH7.4).
translate.label.attr.clone:
A5C6
Conjugate:
Non-conjugated
Immunogen:
Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell.
Isotype:
IgG1
Molecular Weight:
35.7 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
The binding activity of HA600012 with Recombinant TROP2 protein. Immobilized Recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃; and incubated with the primary antibody (HA600012) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600012 is 5.574 ng/ml.
Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600012 at 1/200 dilution (red) compared with Purified human IgG; Isotype Control (black). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.