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Antibodies

Cyclin A1 Rabbit polyclonal Antibody

Product Sizes
100μl
£370.00
HA500287-100UL
About this Product
SKU:
HA500287
Application:
Western Blot, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Cyclin A1 aa 80-300 / 465.
Isotype:
IgG
Molecular Weight:
52/47 kDa
Physical State:
Liquid
Purity:
Immunogen affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Polyclonal Antibody
Western blot analysis of Cyclin A1 on rat testis tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500287; 1/1;000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of Cyclin A1 was done on SH-SY5Y cells. The cells were fixed; permeabilized and stained with the primary antibody (HA500287; 1ug/ml) (red) compared with Rabbit IgG; monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour; the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1;000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).