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Antibodies

Cullin 2 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
ET7108-47-100UL
About this Product
SKU:
ET7108-47
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JG72-31
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within N-terminal Human Cullin 2 .
Isotype:
IgG
Molecular Weight:
89 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Cullin 2 on different lysates with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/500 dilution. Lane 1: PC-3M cell lysate Lane 2: K562 tissue lysate (20 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-47) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET7108-47) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET7108-47) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.