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Antibodies

Phospho-RSK1 (S380) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
ET1610-28-100UL
About this Product
SKU:
ET1610-28
Application:
Western Blot, Immunoprecipitation
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SC05-32
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding S380 of human RSK1.
Isotype:
IgG
Molecular Weight:
Predicted band size: 83 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-RSK1 (S380) on different lysates with Rabbit anti-Phospho-RSK1 (S380) antibody (ET1610-28) at 1/500 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela (starved overnight) then treated with TPA (200nM 4h) cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: NIH/3T3 (starved 24h) then Calyculin A (100nM 15min) cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-28) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature.
ICC staining of Phospho-RSK1 (S380) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-RSK1 (S380) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-RSK1 (S380) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-28; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).