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Antibodies

CD11c Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
ET1606-19-100UL
About this Product
SKU:
ET1606-19
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SI19-06
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human CD11c aa 1;114-1;163 / 1;163 (Cytoplasmic).
Isotype:
IgG
Molecular Weight:
Predicted band size: 128 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Fluorescence multiplex immunohistochemical analysis of the human cervical cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD57 (HA601114; red); anti-CD11c (ET1606-19; green); anti-CD117 (HA21154; magenta) and anti-CD66b (HA500100; yellow) on human cervical cancer. Panel B: anti- CD57 stained on NKT cells. Panel C: anti-CD11c stained on dendritic cells. Panel D: anti-CD117 stained on mast cells. Panel E: anti-CD66b stained on neutrophils. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in four rounds of staining: in the order of HA601114 (1/500 dilution); ET1606-19 (1/1;000 dilution); HA721154 (1/1;000 dilution); and HA500100 (1/1;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (HA601115; Red); anti-CD38 (HA721268; Green); anti-CD23 (HA721139; White); anti-CD11C (ET1606-19; Cyan); anti-CD45 (ET7111-03; Magenta) and anti-CD20 (HA721138; Yellow) on tonsil. Panel B: anti-CD68 stained on Macrophage. Panel C: anti-CD38 stained on lymphocyte subsets. Panel D: anti-CD11C stained on dendritic cells. Panel E: CD45 stained on lymphocytes. Panel F: anti-CD20 stained on B cells. Panel G: anti-CD23 stained on follicular dendritic cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in six rounds of staining: in the order of HA601115 (1/2;000 dilution); HA721268 (1/1;000 dilution); ET1606-19 (1/1;000 dilution); ET7111-03 (1/500 dilution); HA721138 (1/2;000 dilution) and HA721139 (1/800 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Fluorescence multiplex immunohistochemical analysis of human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD45 (ET7111-03; Yellow); anti-CD15 (HA721246; Green) and anti-CD11c (ET1606-19; Red) on tonsil. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET7111-03 (1/500 dilution); HA721246 (1/500 dilution) and ET1606-19 (1/1;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Rabbit anti-CD11c antibody (ET1606-19) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (ET1606-19) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.