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Antibodies

Phospho-EIF2S1 (S51) Recombinant Rabbit monoclonal Antibody

Product Sizes
50uL
£245.00
ET1603-14-50UL
About this Product
SKU:
ET1603-14
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SZ01-06
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser51 of Human eIF-2a.
Isotype:
IgG
Molecular Weight:
Predicted band size: 36 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-EIF2S1 (S51) on different lysates with Rabbit anti-Phospho-EIF2S1 (S51) antibody (ET1603-14) at 1/2;000 dilution. Lane 1: HeLa whole cell lysate (15 µg/Lane) Lane 2: HeLa treated with 50nM Calyculin A for 3 hours whole cell lysate (15 µg/Lane) Lane 3: NIH/3T3 whole cell lysate (15 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (15 µg/Lane) Lane 5: C6 whole cell lysate (20 µg/Lane) Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: Lane 1-4: 2 minutes; Lane 5-6: 23 seconds； 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-14) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-EIF2S1 (S51) on THP-1 cell lysates. Lane 1: THP-1 cells; whole cell lysate; 10ug/lane Lane 2: THP-1 cells treated with 2.8ug/ul lambda-PP for 30 minutes; whole cell lysates; 10ug/lane All lanes : Anti-Phospho-EIF2S1 (S51) antibody (ET1603-14) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10;000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200;000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds
Western blot analysis of Phospho-EIF2S1 (S51) on C2C12 cell lysates. Lane 1: C2C12 cells; whole cell lysate; 10ug/lane Lane 2: C2C12 cells treated with 300nM thapsigargin for 30 minutes; whole cell lysates; 10ug/lane All lanes : Anti-Phospho-EIF2S1 (S51) antibody (ET1603-14 ) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10;000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200;000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds
Western blot analysis of Phospho-EIF2S1 (S51) on mouse spleen tissue lysates. Lane 1: mouse spleen tissue; whole tissue lysate; 20ug/lane Lane 2: mouse spleen tissue treated with 2.8ug/ul lambda-PP for 30 minutes; whole tissue lysates; 20ug/lane All lanes : Anti-Phospho-EIF2S1 (S51) antibody (ET1603-14) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10;000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200;000 dilution. Predicted band size: 36 kDa Observed band size: 36 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds