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Antibodies

LGR5 Rabbit polyclonal Antibody

Product Sizes
100μl
£370.00
ER10708-100UL
About this Product
SKU:
ER10708
Application:
Western Blot
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to Mouse GPR49 / LGR5 alpha / beta aa 1-50 / 907 (ID: Q9Z1P4).
Isotype:
IgG
Molecular Weight:
Predicted band size: 100 kDa
Physical State:
Liquid
Purity:
Immunogen affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Polyclonal Antibody
Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (ER10708) at 1/1;000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER10708) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of LGR5 on different cell lysates using anti- GPR49/LGR5 alpha/beta antibody at 1/1;000 dilution. Positive control: Lane 1: F9 cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate