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Antibodies

CRISPR-Cas9 Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM50804-100UL
About this Product
SKU:
EM50804
Application:
Western Blot
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
8-A6-6
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Streptococcus Pyogenes CRISPR-Cas9 aa1-200.
Isotype:
IgM
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5;000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate
Immunofluorescence staining CRISPR-Cas9 in CRISPR-Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry analysis of 293T cells transfected with or without px459 labeling CRISPR-Cas9 with Mouse anti-CRISPR-Cas9 antibody (EM50804) at 1/1;000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CRISPR-Cas9 antibody (EM50804) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) were used as the secondary antibody at 1/1;000 dilution.