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Antibodies

CD11b Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM1902-35-100UL
About this Product
SKU:
EM1902-35
Application:
Western Blot
Buffer:
1*TBS (pH7.4); 1%BSA; 50%Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A2-B5-D2
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within mouse CD11b aa 1-300.
Isotype:
IgM
Molecular Weight:
Predicted band size: 127 kDa.
Physical State:
Liquid
Purity:
Protein A/G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution. Lane 1: RAW264.7 cell lysate (RIPA lysis) Lane 2: RAW264.7 cell lysate (hot lysis) Lane 3: RAW264.7 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35; 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.