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Antibodies

Cytokeratin 20 Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM1901-96-100UL
About this Product
SKU:
EM1901-96
Application:
Western Blot, Flow Cytometry, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A3D12
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide within Human Cytokeratin 20 aa 29-78 / 424.
Isotype:
IgG1
Molecular Weight:
48 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Cytokeratin 20 on Lovo cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-96; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1901-96; 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1901-96; 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1901-96; 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.