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Antibodies

HLA Class 1 ABC Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM1801-10-100UL
About this Product
SKU:
EM1801-10
Application:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
11C2
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human HLA Class 1 B aa 1-362 / 362.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 41 kDa
Physical State:
Liquid
Purity:
Immunogen affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 2 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/2;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-HLA Class 1 ABC KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/2;000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1801-10) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1801-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.