Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

IMP3 Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM1801-01-100UL
About this Product
SKU:
EM1801-01
Application:
Western Blot, Flow Cytometry
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
4-C2-5
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human IMP3 aa 350-579.
Isotype:
IgG
Molecular Weight:
Predicted band size: 64 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/2;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Mouse placenta tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Lane 7: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: Lane 1-4: 20 seconds; Lane 5-6: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/5;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of PANC-1 cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.