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Antibodies

GRB2 Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
EM1701-41-100UL
About this Product
SKU:
EM1701-41
Application:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 50% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
11-B11
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human GRB2 aa 21-217 / 217.
Isotype:
IgG2b
Molecular Weight:
Predicted band size: 25 kDa
Physical State:
Liquid
Purity:
Protein G affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of GRB2 on different lysates with Mouse anti-GRB2 antibody (EM1701-41) at 1/1;000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: A431 cell lysate (30 µg/Lane) Lane 3: NIH/3T3 cell lysate (30 µg/Lane) Lane 4: PC-12 cell lysate (30 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-41) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-GRB2 antibody (EM1701-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1701-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-GRB2 antibody (EM1701-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (EM1701-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin.