Anti-GFP Purified
Product Sizes
0.025 mg
£121.00
11-476-0.025MG
0.1 mg
£202.00
11-476-0.1MG
About this Product
- SKU:
- 11-476
- Application:
- Immunocytochemistry, Immunoprecipitation, Western Blot
- CE/IVD:
- RUO
- translate.label.attr.clone:
- PAb (476)
- Concentration:
- 1 mg/ml
- Extra Details:
- Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (L Lambda=509 nm) when excited (excitation by Blue or UV light, absorption peak L Lambda=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. yellow fluorescent protein, cyan fluorescent protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
- Immunogen:
- EGFP, a native full-length protein
- Shipping Conditions:
- Blue Ice
- Specificity:
- The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
- Storage Conditions:
- 2-8[o]C Do not freeze.
- Supplier:
- Exbio
- Type:
- Antibody
