Anti-Zebrafish MCM2 Antibody Picoband®
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Anti-Zebrafish MCM2 Antibody Picoband®
£ POA
AZA0A0R4Immunofluorescence65
About this Product
- SKU:
- AZA0A0R4Immunofluorescence65
- Application:
- Western Blot
- Buffer:
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
- Clonality:
- Polyclonal
- Concentration:
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Extra Details:
- Boster Bio Anti-Zebrafish MCM2 Antibody Picoband® catalog #AZA0A0R4Immunofluorescence65. Tested in Western Blot applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Host:
- Rabbit
- Immunogen:
- E.coli-derived zebrafish MCM2 recombinant protein (Position: A381-R835)
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
- Supplier:
- Boster Bio
- Manufacturer's Data Sheet:datasheet
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (AZA0A0R4Immunofluorescence65) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125 kDa. The expected band size for MCM2 is at 102 kDa.
