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Antibodies

CD28 Antibody

Product Sizes
10 ug
£214.00
ORB757386-10UG
50 ug
£502.00
ORB757386-50UG
100 ug
£818.00
ORB757386-100UG
500 ug
£2210.00
ORB757386-500UG
About this Product
SKU:
ORB757386
Application:
ELISA, Flow Cytometry
translate.label.attr.clone:
DM63
Clonality:
Monoclonal
Conjugate:
Unconjugated
Format:
Liquid
Host:
Rabbit
Isotype:
Rabbit IgG
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies: Monoclonal Antibody
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD28 protein; mFc-His tagged protein orb689375 can bind Rabbit anti-CD28 monoclonal antibody (clone: DM63) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1 μg/ml (clone: DM63) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM63) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human CD28 mAb (orb757386). (A) orb757386 does not bind to 293T cells that do not express CD28. (B) A clear peak shift of orb757386 was seen compared to the control when incubated with CD28-expressing Jurkat cells; indicating strong binding of orb757386 to CD28. Antibodies were incubated at 2 μg/mL.