Biorbyt
Their mission today is to provide the gold standard of service to the global scientific community, by collaborating with the world’s best researchers and academics, together creating and distributing the most reliable and highest quality products possible. Through precision manufacturing and rigorous testing, they can supply the widest possible range of reagents on the market - including those which many of our competitors cannot offer.
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Custom Services
Biorbyt offer a range of services to enable researchers to access the products they need, but that might not yet be offered as off the shelf products.
Polyclonal antibodies
- Lead time 12-17 weeks
- QC report of antigen
- WB Guarantee for antigen
- 1ml pre-immune serum, 2ml antiserum
- 90% antibody purity by SDS-PAGE detection
Monoclonal Antibodies
- Lead time 20-25 weeks
- QC report of antigen
- 100ul x ascites
- ELISA titre guarantee 1:10000
- WB guarantee for antigen
- Validation report of the hybridoma
- Other applications validation available upon request (IHC, IF, IP, FC)
Recombinant antibodies
- Lead time 12-16 weeks
- QC report of antigen
- 100ug of purified recombinant antibody with QC report (Includes gene information, gene sequence, concentration, purity, endotoxin content)
- WB positive guarantee for antigen
- Other applications validation available upon request (IHC, IF, IP, FC)
Antibody conjugation
Biorbyt offer a range of custom conjugations on many of the antibodies it the range. These are some of the regularly offered options:-
Conjugation | Abs/Em Maxima | Excitation Laser Line | Dye Profile | Other Details |
Biotin | N/A | N/A | N/A | Can be used in a variety of assays, including ELISA and Western blotting |
FITC | 494/518 nm | 488 nm | A popular choice for flow cytometry and immunofluorescence microscopy | |
CF 405M | 408/452 nm | 405 nm | A good choice for multicolour flow cytometry | |
CF 488A | 490/515 nm | 488 nm | A brighter alternative to FITC | |
CF 594 | 593/614 nm | 561 to 568 nm | A good choice for multicolour flow cytometry | |
CF 647 | 650/665 nm | 633/635/640 nm | A good choice for multicolour flow cytometry |
Not all antibodies are suitable for conjugation
Recombinant Protein expression
With a range of different expression systems available, options on post translational modifications, protein activity requirements and scalability can be selected so there’s a system to suit your needs! With 39 different tags to choose from and quantities from 10mg to 200mg and additional services such as desalination, endotoxin elimination and lyophilization offered on a complimentary basis.
Peptide synthesis
For peptides up to 100aa long, the standard offering is to produce 5-50mg, although bulk production can be accommodated.
Options on your peptide include:-
- N-terminal, c-terminal and side chain modifications
- D-amino acid and stable-isotope incorporation
- Biotinylation
- Fluorescent labels
- Post translational modifications
- Multiple disulphide bridges
- Glycosylation
- PEGylation
Peptide and biochemical purity are assessed through a variety of methods, but additional amino acid analysis or microanalysis can be ordered.
For all Biorbyt’s custom services contact the Product Manager team to get started!