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Antibodies

ATF4 Recombinant Rabbit Monoclonal Antibody

Product Sizes
50 ul
£381.00
ORB704304-50UL
100 ul
£530.00
ORB704304-100UL
About this Product
SKU:
ORB704304
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
9C3
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
A synthesized peptide derived from human ATF4 (1-220/351aa)
Isotype:
IgG
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ATF4 antibody (orb704304); dilution: 1:50; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488; dilution: 1:1000. Protocol; The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
N2A cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ATF4) monoclonal Antibody; Unconjugated (orb704304) 1:50; 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes; DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed; paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATF4) Monoclonal Antibody; Unconjugated (orb704304) at 1:50 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATF4) Monoclonal Antibody; Unconjugated (orb704304) at 1:50 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATF4) Monoclonal Antibody; Unconjugated (orb704304) at 1:50 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.