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Antibodies

Phospho-HSF1 (Ser326) Recombinant Rabbit Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB559454-25UL
50 ul
£381.00
ORB559454-50UL
100 ul
£530.00
ORB559454-100UL
200 ul
£660.00
ORB559454-200UL
200 ug
£882.00
ORB559454-200UG
About this Product
SKU:
ORB559454
Application:
Flow Cytometry, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
37E4
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326 L(p-S)PT
Isotype:
IgG
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-HSF1 (Ser326) antibody (orb559454); dilution: 2 µg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488; dilution: 1 µg/Test. Protocol; The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed; paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-HSF1 (Ser326)) Monoclonal Antibody; Unconjugated (orb559454) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-HSF1 (Ser326)) Polyclonal Antibody; Unconjugated (orb559454) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-HSF1 (Ser326)) Polyclonal Antibody; Unconjugated (orb559454) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-HSF1 (Ser326)) Polyclonal Antibody; Unconjugated (orb559454) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.