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Antibodies

Phospho-HSP27 (Ser78) Recombinant Rabbit Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB559187-25UL
50 ul
£381.00
ORB559187-50UL
100 ul
£530.00
ORB559187-100UL
About this Product
SKU:
ORB559187
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
5E1
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78 AL(p-S)RQ
Isotype:
IgG
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
Blocking buffer: 5% NFDM/TBST; Primary Ab; dilution: 1:1000; Primary Ab incubation condition: 2 hours at room temperature; Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP); Lysate: 1: HeLa + Anisomycin (-); 2 HeLa +; Anisomycin (+); Protein loading quantity: 20 µg; Exposure time: 60 s; Predicted MW: 23 kDa; Observed MW: 27 kDa.
Cell line: A: HeLa; B HeLa + Anisomycin (+); Fixation: 100% Ice-cold methanol; Permeabilization: 0.1% Triton X-100; Primary Ab; dilution: 1:50; Primary Ab incubation condition: 4°C overnight; Secondary Ab: Goat Anti-Rabbit IgG; Nuclear counter stain: DAPI (Blue); Comment: Color green is the positive signal for orb559187.
Tissue: Human breast cancer; Section type: Formalin-fixed & Paraffin embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0; Primary Ab; dilution: 1:500; Primary Ab incubation condition: 1 hour at room temperature; Secondary Ab: Anti-Rabbit and Mouse; Polymer HRP (Ready to use); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb559187.
Tissue: Human cervical cancer; Section type: Formalin-fixed & Paraffin embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0; Primary Ab; dilution: 1:500; Primary Ab incubation condition: 1 hour at room temperature; Secondary Ab: Anti-Rabbit and Mouse; Polymer HRP (Ready to use); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb559187.
Tissue: Human tonsil; Section type: Formalin-fixed & Paraffin embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0; Primary Ab; dilution: 1:500; Primary Ab incubation condition: 1 hour at room temperature; Secondary Ab: Anti-Rabbit and Mouse; Polymer HRP (Ready to use); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb559187.