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Antibodies

Alpha Synuclein Antibody (APC)

Product Sizes
100 ug
£688.00
ORB413361-100UG
About this Product
SKU:
ORB413361
Application:
Dot Blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
95.46mM Phosphate; 2.48mM MES and 2mM EDTA
translate.label.attr.clone:
4F1
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Host:
Mouse
Immunogen:
Mouse alpha synuclein aggregate
Isotype:
IgG1
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Conjugated antibodies should be stored according to the product label
Supplier:
Biorbyt
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody; Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min at RT; 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
Western Blot analysis of Human; Mouse; Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody; Clone 4F1. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band (s): ~40 kDa (trimer).
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody; Clone 4F1. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate at 4 μg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:200 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000; 1:3000 for 60 min at RT; 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) Alpha Synuclein Antibody. (D) Composite.