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Antibodies

Anti-CD3 epsilon [YTH 12.5]

Product Sizes
0.2 mg
£632.00
ORB411552-0.2MG
About this Product
SKU:
ORB411552
Application:
Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS with 0.02% Proclin 300.
translate.label.attr.clone:
YTH 12.5
Clonality:
Monoclonal
Conjugate:
Unconjugated
Concentration:
1 mg/ml
Host:
Rat
Immunogen:
Human CD3.
Isotype:
Rat IgG
Purity:
Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies: Monoclonal Antibody
Flow-cytometry using anti-CD3 antibody YTH 12.5 Rhesus monkey lymphocytes were stained with an isotype control (orb256390; red) or the rabbit-chimeric version of YTH 12.5 (orb411552; blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing; bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.
Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5 Human lymphocytes were stained with an isotype control (orb256390; panel A) or the rabbit-chimeric version of YTH 12.5 (orb411552; panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing; bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow-cytometry using the anti-CD3E antibody YTH 12.5 Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTH 12.5 (orb411552; blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing; bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.
Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody YTH 12.5 Anti-CD3E staining of paraffin embedded human tissue using the rabbit-chimeric version of YTH 12.5 (orb411552). Antigen retreival was acheived by microwaving in citrate buffer (pH6); followed by blocking with protein block serum-free buffer. Primary antibody incubation with orb411552 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3; 3′-diaminobenzidine); and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration; 2-4 µg/ml.
Western Blot using anti-CD3E antibody YTH 12.5 Human spleen sample (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 12.5 (orb411552) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for CD3E is 23.1 kDa. orb411552 successfully detected human CD3E.