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Antibodies

LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB2563049-25UL
50 ul
£381.00
ORB2563049-50UL
100 ul
£530.00
ORB2563049-100UL
About this Product
SKU:
ORB2563049
Application:
Flow Cytometry, Western Blot
Buffer:
0.01M TBS(pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
8H13
Clonality:
Recombinant
Conjugate:
Unconjugated
Format:
Liquid
Host:
Rabbit
Immunogen:
A synthesized peptide derived from human LGR5 (818-907aa)
Isotype:
IgG
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
25 ug total protein per lane of various lysates probed with LGR5/GPR49 monoclonal antibody; unconjugated (orb2563049) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C; the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-LGR5/GPR49 antibody (orb2563049): 1 µg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.