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Antibodies

Caspase 3 Antibody

Product Sizes
30 ul
£196.00
ORB213646-30UL
50 ul
£224.00
ORB213646-50UL
100 ul
£307.00
ORB213646-100UL
200 ul
£400.00
ORB213646-200UL
About this Product
SKU:
ORB213646
Application:
Immunofluorescence, Immunohistochemistry, Western Blot
Clonality:
Polyclonal
Conjugate:
Unconjugated
Format:
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.
Host:
Rabbit
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
Reactivities:
Canine, Human, Non-human Primate, Mouse, Porcine, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies: Polyclonal Antibody
Western blot analysis of Caspase 3 expression in SGC7901 (A); mouse kidney (B); A549 (C); Hela (D); LO2 (E) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 35 kD)
Immunohistochemical analysis of Caspase 3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Caspase 3 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).