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Antibodies

ADGRE1 Recombinant Rabbit Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB1974665-25UL
50 ul
£381.00
ORB1974665-50UL
100 ul
£530.00
ORB1974665-100UL
200 ul
£660.00
ORB1974665-200UL
200 ug
£882.00
ORB1974665-200UG
About this Product
SKU:
ORB1974665
Application:
Flow Cytometry, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
7E2
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant human ADGRE1 (1-530/886aa)
Isotype:
IgG
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
50 ng rhEMR1-His protein per lane probed with ADGRE1 polyclonal antibody respectively; unconjugated (orb1974665) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody (orb1974665); dilution: 1 ug/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC; dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG; Protocol; The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Human THP-1 cell lysates; Primary: Anti-ADGRE1 (orb1974665) at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 95 kDa; Observed band size: 100 kDa.