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Antibodies

CD28 Rabbit Polyclonal Antibody

Product Sizes
50 ul
£307.00
ORB1974617-50UL
100 ul
£437.00
ORB1974617-100UL
200 ul
£511.00
ORB1974617-200UL
About this Product
SKU:
ORB1974617
Application:
Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS(pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
Clonality:
Polyclonal
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant human CD28 protein (19-152/220aa)
Isotype:
IgG
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies: Polyclonal Antibody
Paraformaldehyde-fixed; paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD28 Rabbit pAb) Polyclonal Antibody; Unconjugated (orb1974617) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Recombinant human CD28 protein; C-hFc (HEK193); Primary: Anti-CD28 (orb1974617) at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 25 kDa; Observed band size: 60 kDa.