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Alpha Synuclein A53T Mutant Pre-formed Fibrils

Product Sizes
100 ug
£688.00
ORB1822360-100UG
About this Product
SKU:
ORB1822360
Application:
In Vivo Administration, SDS-PAGE, Western Blot
Buffer:
PBS pH 7.4
Concentration:
2 mg/ml
Purity:
>95%
Reactivities:
Human
Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Shipping Conditions:
Dry Ice
Source:
Recombinant
Storage Conditions:
-80°C
Supplier:
Biorbyt
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules
Primary rat hippocampal neurons show lewy body inclusion formation when treated with A53T mutant Alpha Synuclein Protein Pre-formed Fibrils (B) but not when treated with a media control (A). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Primary Antibody: Rabbit anti-pSer129 Antibody. Fibrils were diluted to 1 ug/uL in neuronal media consisting of B27; Glutamax; penicillin/strip; and neurobasalA and sonicated for 1 hour in a water bath. The sonicated stock was then used to achieve the final concentration of 1 ug/mL in the wells.
TEM of A53T alpha synuclein Pre-formed Fibrils
TEM of A53T alpha synuclein Pre-formed Fibrils
A53T alpha synuclein monomers and fibrils are well recognized by homogeneous time-resolved fluorescence (HTRF) total alpha synuclein assay.
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding; the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers. A much greater increase in fluorescence is seen when 100 μM monomer is combined with 10 μM of fibrils as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm; em = 485 nm.