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Antibodies

GFAP Antibody (APC)

Product Sizes
100 ug
£688.00
ORB150542-100UG
About this Product
SKU:
ORB150542
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
95.46mM Phosphate; 2.48mM MES and 2mM EDTA
translate.label.attr.clone:
N206B/9 (Formerly sold as S206B-9)
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Host:
Mouse
Immunogen:
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. > 50% identity with other proteins (Vimentin; Desmin and Peripherin).
Isotype:
IgG1
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Conjugated antibodies should be stored according to the product label
Supplier:
Biorbyt
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GFAP R416WT Monoclonal Antibody; Clone N206B/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GFAP R416WT Monoclonal Antibody at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800; 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GFAP R416WT Antibody (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of GFAP protein using Mouse Anti-GFAP Monoclonal Antibody; Clone N206B/9. Primary Antibody: Mouse Anti-GFAP Monoclonal Antibody at 1:250.