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Antibodies

Phospho-Histone H3 (Ser10) Recombinant Mouse Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB1172832-25UL
50 ul
£381.00
ORB1172832-50UL
100 ul
£530.00
ORB1172832-100UL
About this Product
SKU:
ORB1172832
Application:
Immunocytochemistry, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
C10C6
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Mouse
Immunogen:
KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10 RK(p-S)TG
Isotype:
IgG1; Kappa
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
Blocking buffer: 5% NFDM/TBST; Primary ab Dilution: 1:1000; Primary ab incubation condition: 2 hours at room temperature; Secondary ab: Goat Anti-Mouse IgG H&L (HRP); Lysate: (-) HeLa; (+) HeLa + Nocodazole (100 ng/ml; 18hr); Protein loading quantity: 20 µg; Exposure time: 3 s; Predicted MW: 15 kDa; Observed MW: 15 kDa.
Tissue: Human kidney; Section type: Formalin fixed & Paraffin-embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0 Primary ab Dilution: 1:100; Primary ab incubation condition: 1 hour at room temperature; Secondary ab: SP Kit (Mouse); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb1172832.
Tissue: Mouse liver; Section type: Formalin fixed & Paraffin-embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0 Primary ab Dilution: 1:100; Primary ab incubation condition: 1 hour at room temperature; Secondary ab: SP Kit (Mouse); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb1172832.
Tissue: Rat testis; Section type: Formalin fixed & Paraffin-embedded section; Retrieval method: High temperature and high pressure; Retrieval buffer: Tris/EDTA buffer; pH9.0 Primary ab Dilution: 1:100; Primary ab incubation condition: 1 hour at room temperature; Secondary ab: SP Kit (Mouse); Counter stain: Hematoxylin (Blue); Comment: Color brown is the positive signal for orb1172832.