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Antibodies

ALDH1A1 Recombinant Rabbit Monoclonal Antibody

Product Sizes
25 ul
£242.00
ORB1152015-25UL
50 ul
£381.00
ORB1152015-50UL
100 ul
£530.00
ORB1152015-100UL
About this Product
SKU:
ORB1152015
Application:
Immunofluorescence, IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
0.01M TBS (pH7.4) with 1% rAlbumin; 0.02% Proclin300 and 50% Glycerol.
translate.label.attr.clone:
AH12
Clonality:
Recombinant
Conjugate:
Unconjugated
Concentration:
1mg/ml
Format:
Liquid
Host:
Rabbit
Immunogen:
KLH conjugated synthetic peptide derived from human ALDH1A1
Isotype:
IgG
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Storage Conditions:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier:
Biorbyt
Type:
Antibodies
Blocking buffer: 5% NFDM/TBST; Primary ab Dilution: 1:2000; Primary ab incubation condition: 2 hours at room temperature; Lysate: HepG2; Raji; Jurkat; K562; A549; Mouse liver; Protein loading quantity: 20 µg; Exposure time: 10 s; Predicted MW: 55 kDa; Observed MW: 55 kDa.
Paraformaldehyde-fixed; paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody; Unconjugated (orb1152015) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody; Unconjugated (orb1152015) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody; Unconjugated (orb1152015) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed; paraffin embedded (human liver); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ALDH1A1) Monoclonal Antibody; Unconjugated (orb1152015) at 1:200 overnight at 4°C; followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.