THUNDER Extreme Phospho-ERK1/2 (T202/Y204) + Total ERK TR-FRET Cell Signaling Assay Kit
Product Sizes
500 points (400 points for phospho; 100 points for total)
£1289.00
KIT-XTM-ERKPT-500-500POINTS
About this Product
- SKU:
- KIT-XTM-ERKPT-500
- Application:
- Cell-based/Functional Assay
- Extra Details:
- Highly sensitive sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-ERK1/2 (T202/Y204) in cell lysates. This kit is suitable for measuring the inhibition of basal phospho-ERK1/2 levels
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- at -80oC
- Supplier:
- BioAuxilium
- Type:
- Assay Kits
- Kit complementary reagents:
- ERK Control lysate: LYS-ERK-100 (100 µL) LYS-ERK-500 (500 µL) Lysis Bufer #1 (5X; 10 mL): TRFRET-LB1 Detection Buffer (10X; 1 mL): TRFRET-DB
- Assay type:
- Semi-quantitative; homogeneous (no-wash) sandwich immunoassay
- Assay format:
- Half-area 96-well or low-volume 384-well plate
- Assay target:
- Phospho-ERK1/2 (T202/Y204) and Total ERK1/2
- Components:
- Eu-phospho-ERK (20 µL) Acc-phospho-ERK (80 µL) Eu-Total ERK (5 µL) Acc-Total ERK (20 µL) ERK control lysate (200 µL) 5X Lysis Buffer #1 (5 mL) 10X TR-FRET Detection buffer (250 µL) 100X Phosphatase Inhibitor Cocktail (250 µL)
