BioAuxilium
BioAuxilium is a Canadian biotechnology company founded by seasoned scientists from PerkinElmer. It focuses on the design, development and manufacturing of top-quality, fully-validated assay kits that simplify laboratory workflow and accelerate biomedical research. Since its inception in 2013, Bioauxilium has licensed hundreds of TR FRET assay kits enabling the quantification of cellular proteins and biomarkers. BioAuxilium’s kits are based on its proprietary THUNDER™ TR-FRET technology. They are offered at an outstanding value and are supported by expert technical support.
View All BioAuxilium Products
THUNDER cAMP TR-FRET Assay Kit
The THUNDER™ TR-FRET cAMP Detection Kit offers a reliable and efficient method for quantitatively measuring cyclic AMP (cAMP) in cell lysates from both suspension and adherent cells. Ideal for characterisation and high-throughput screening of Gs- and Gi-coupled GPCRs, the kit ensures high sensitivity and accuracy with a leading TR-FRET performance.
The kit features a straightforward two-step protocol and all necessary components including: cAMP standard, biotin-cAMP, Eu-streptavidin, Acc-anti-cAMP, and 5X cAMP Detection Buffer. Its competitive immunoassay relies on free cAMP competing with a Europium-labelled streptavidin/biotin-cAMP tracer for binding to a far-red fluorophore-labelled monoclonal cAMP antibody, with the TR-FRET signal inversely proportional to the cAMP concentration to provide accurate results to your valuable research.
THUNDER Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit
Enhance your research with the THUNDER™ Phospho-STAT (Signal Transducer and Activator of Transcription) TR-FRET Assay Kit, designed for precise cell-based assays in drug discovery and advanced research. Available for Phospho-STAT 1, 3, 5, and 6, this impressive kit offers reliable and reproducible results with consistent performance.
Utilising BioAuxilium’s TR-FRET technology, the THUNDER™ kit allows precise detection of phosphorylated STAT in cell lysates. It features Europium-labelled and acceptor-labelled antibodies that bind to different epitopes on STAT. Excitation of the Europium donor yields a FRET emission at 665 nm, directly measuring Phospho-STAT levels.
There are a range of benefits from choosing BioAuxilium’s Phospho-STAT kit include high sensitivity, rapid results, and consistent lot-to-lot performance in human and mouse cells. Overall resulting in a reliable, cost-effective, and multi-format solution, that is compatible with all TR-FRET readers.
If you require further measurement of various aspects of protein phosphorylation and expression, please take a look at the kits below!
• Total Protein Kits: Assess total proteins, useful for normalising data or monitoring expression levels.
• Phospho + Total Protein Kits: Detect phosphorylated and total proteins simultaneously in separate wells.
BioAuxilium Product Categories | ||
More information about BioAuxilium
BioAuxilium founders have over 20 years of academic and industry experience. They have a renowned expertise in the design, development and manufacturing of assay kits and reagents on the most popular technology platforms available (e.g., TR-FRET, AlphaScreen®/AlphaLISA®, BRET/BRET2, FP, Aequorin and more).
BioAuxilium is based in Montréal (Canada) and located within NEOMED’s state-of-the-art laboratories and core facilities where the company provides a wide range of product and assay development services to academic and industry research groups.
THUNDERTM TR-FRET Human Cytokine Assay Kits - The real alternative to Elisa and existing TR-FRET assays
BioAuxilium has expanded its line of THUNDER™ TR-FRET cell-based kits with the launch of assays designed to quantitate key pro-inflammatory human cytokines in cell supernatants in a simple, quick, reproducible and robust manner. The new kits contain
matched labelled-antibody pairs and an optimized assay buffer to develop a TR-FRET immunoassay using the recommended standard, which must be purchased separately. They offer an economical and versatile alternative to buying complete kits.
A DIRECT COMPARISON OF THUNDER™ ASSAY PERFORMANCE WITH TWO EXISTING TR-FRET PLATFORMS
We compared head- to-head the performance of THUNDER™ with two existing TR-FRET assay technologies. Six endogenous phosphorylated proteins were measured in lysates from cells treated with pathway-specific modulators.
THUNDER™ phospho-protein assays exhibit superior or comparable performance at a lower cost per well.
BioAuxilium has recently introduced THUNDER™, a new immunoassay platform based on an enhanced TR-FRET technology. The THUNDER™ Cell Signaling Assay Kits are designed to measure endogenous levels of specific intracellular phosphorylated proteins with high sensitivity, specificity, robustness and cost effectiveness. The aim of this Application Note was to compare head-to-head the performance of THUNDER™ in 384-well plate format with two existing TR-FRET technologies (Companies A and B) measuring a panel of six phosphorylated proteins. The three TR-FRET assay technology
platforms were evaluated for their capacity to measure relative levels of phosphorylated 4EBP1 (T37/T46), AKTpan (S473), ERK1/2 (T202/ Y204) p38αβγ (T180/Y182), SLP-76 (S376) and STAT3 (Y705) in wholecell lysates from cells treated with pathway-specific modulators.
New THUNDER™ TR-FRET Toolbox Reagents
BioAuxilium have released their new range of products -THUNDER™ TR-FRET Toolbox Reagents!
These reagents are used by drug discovery scientists to design TR-FRET assays using anti-tag and/or streptavidin labelled with either the donor (Europium chelate) or the acceptor (far-red dye) fluorophores.
