THUNDER Phospho-RPS6 (S240/S244) + Total RPS6 TR-FRET Cell Signaling Assay Kit
Product Sizes
500 points (400 points for phospho; 100 points for total)
KIT-RPS6BPT-500-500POINTS
About this Product
- SKU:
- KIT-RPS6BPT-500
- Application:
- Cell-based/Functional Assay
- Extra Details:
- Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Ribosomal Protein S6 (S240/S244) and Total Ribosomal Protein S6 in cell lysates
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- at -80oC
- Supplier:
- BioAuxilium
- Type:
- Assay Kits
- Kit complementary reagents:
- RPS6 Control lysate: LYS-RPS6-100 (100 µL) LYS-RPS6-500 (500 µL) Lysis Bufer #4 (5X; 10 mL): TRFRET-LB4 Detection Buffer (10X; 1 mL): TRFRET-DB
- Assay type:
- Semi-quantitative; homogeneous (no-wash) sandwich immunoassay
- Assay format:
- Half-area 96-well or low-volume 384-well plate
- Assay target:
- Phospho-Ribosomal Protein S6 (S240/244) and Total Ribosomal Protein S6
- Components:
- Eu-phospho-RPS6S240 (20 µL) Acc-phospho-RPS6S240 (80 µL) Eu-Total RPS6 (5 µL) Acc-Total RPS6 (20 µL) RPS6 control lysate (200 µL) 5X Lysis Buffer 4 (5 mL) 10X TR-FRET Detection buffer (250 µL)
