THUNDER Phospho-BTK (Y223) + Total BTK TR-FRET Cell Signaling Assay Kit
Product Sizes
500 points (400 points for phospho; 100 points for total)
£1289.00
KIT-BTKPT-500-500POINTS
About this Product
- SKU:
- KIT-BTKPT-500
- Application:
- Cell-based/Functional Assay
- Extra Details:
- Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) and Total BTK in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total BTK proteins from the same lysate sample in separate wells
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- at -80oC
- Supplier:
- BioAuxilium
- Type:
- Assay Kits
- Kit complementary reagents:
- BTK Control lysate: LYS-BTK-100 (100 µL) LYS-BTK-500 (500 µL) Lysis Bufer #2 (5X; 10 mL): TRFRET-LB2 Detection Buffer (10X; 1 mL): TRFRET-DB
- Assay type:
- Semi-quantitative; homogeneous (no-wash) sandwich immunoassay
- Assay format:
- Half-area 96-well or low-volume 384-well plate
- Assay target:
- Phospho-BTK (Y223) & Total BTK
- Components:
- Eu-phospho-BTK (20 µL) Acc-phospho-BTK (80 µL) Eu-Total BTK (5 µL) Acc-Total BTK (20 µL) BTK control lysate (200 µL) 5X Lysis Buffer #2 (5 mL) 10X TR-FRET Detection buffer (250 µL)
