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Proteins and Peptides

TOP3A Recombinant Protein

Product Sizes
10ug
£268.00
OPCD07636-10UG
50ug
£517.00
OPCD07636-50UG
200ug
£929.00
OPCD07636-200UG
About this Product
SKU:
OPCD07636
Additional Names:
DNA topoisomerase 3-alpha;DNA topoisomerase III alpha;MGRISCE2;PEOB5;TOP3;topo III-alpha;topoisomerase (DNA) III alpha;ZGRF7;zinc finger; GRF-type containing 7.
Conjugate:
Unconjugated
Concentration:
200 ug/mL (prior to lyoph)
Extra Details:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme; resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally; in the religation step; the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex; a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
Gene Details:
DNA topoisomerase III alpha
Host:
E. coli
Molecular Weight:
19kDa
Protein Details:
DNA topoisomerase 3-alpha
Purity:
> 95%
Shipping Conditions:
Blue Ice
Source:
E.coli
Storage Conditions:
2Â[o]C to 8Â[o]C|-80Â[o]C
Supplier:
Aviva Systems Biology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins