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Proteins and Peptides

TOP1 Recombinant Protein

Product Sizes
10ug
£272.00
OPCD07631-10UG
50ug
£528.00
OPCD07631-50UG
200ug
£955.00
OPCD07631-200UG
About this Product
SKU:
OPCD07631
Additional Names:
DNA topoisomerase 1;DNA topoisomerase I;Scl-70 antigen;TOPI;topoisomerase (DNA) I;type I DNA topoisomerase.
Conjugate:
Unconjugated
Concentration:
200 ug/mL (prior to lyoph)
Extra Details:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme; resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand; thus removing DNA supercoils. Finally; in the religation step; the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Gene Details:
DNA topoisomerase I
Host:
E. coli
Molecular Weight:
40kDa
Protein Details:
DNA topoisomerase 1
Purity:
> 97%
Shipping Conditions:
Blue Ice
Source:
E.coli
Storage Conditions:
2Â[o]C to 8Â[o]C|-80Â[o]C
Supplier:
Aviva Systems Biology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins