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Proteins and Peptides

TOP3B Recombinant Protein

Product Sizes
10ug
£246.00
OPCD00469-10UG
50ug
£463.00
OPCD00469-50UG
200ug
£825.00
OPCD00469-200UG
About this Product
SKU:
OPCD00469
Additional Names:
DNA topoisomerase 3-beta-1;DNA topoisomerase III beta-1;TOP3B1;topoisomerase (DNA) III beta;topoisomerase III beta.
Conjugate:
Unconjugated
Concentration:
200 ug/mL (prior to lyoph)
Extra Details:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme; resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally; in the religation step; the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Gene Details:
DNA topoisomerase III beta
Host:
E. coli
Molecular Weight:
32kDa
Protein Details:
DNA topoisomerase 3-beta-1
Purity:
> 90%
Shipping Conditions:
Blue Ice
Source:
E.coli
Storage Conditions:
2Â[o]C to 8Â[o]C|-80Â[o]C
Supplier:
Aviva Systems Biology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins