TOP1MT Recombinant Protein
Product Sizes
10ug
£272.00
OPCD00468-10UG
50ug
£528.00
OPCD00468-50UG
200ug
£955.00
OPCD00468-200UG
About this Product
- SKU:
- OPCD00468
- Additional Names:
- DNA topoisomerase 1; mitochondrial;DNA topoisomerase I; mitochondrial;mitochondrial topoisomerase I;topoisomerase (DNA) I; mitochondrial.
- Conjugate:
- Unconjugated
- Concentration:
- 200 ug/mL (prior to lyoph)
- Extra Details:
- Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme; resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand; thus removing DNA supercoils. Finally; in the religation step; the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
- Gene Details:
- DNA topoisomerase I mitochondrial
- Host:
- E. coli
- Molecular Weight:
- 32kDa
- Protein Details:
- DNA topoisomerase I; mitochondrial
- Purity:
- > 90%
- Shipping Conditions:
- Blue Ice
- Source:
- E.coli
- Storage Conditions:
- 2Â[o]C to 8Â[o]C|-80Â[o]C
- Supplier:
- Aviva Systems Biology
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Manufacturer's Data Sheet:html_datasheet.php
