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Proteins and Peptides

RPA1 Recombinant Protein

Product Sizes
20ug
£348.00
OPCA03943-20UG
100ug
£500.00
OPCA03943-100UG
1mg
£1822.00
OPCA03943-1MG
About this Product
SKU:
OPCA03943
Additional Names:
HSSB;MST075;MSTP075;REPA1;replication factor A protein 1;replication protein A 70 kDa DNA-binding subunit;replication protein A1; 70kDa;RF-A;RF-A protein 1;RP-A;RP-A p70;RPA70;single-stranded DNA-binding protein.
Extra Details:
As part of the heterotrimeric replication protein A complex (RPA/RP-A); binds and stabilizes single-stranded DNA intermediates; that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel; recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720; PubMed:27723717). Thereby; it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage; the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP; which is involved in replication fork restart; to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex; aRPA; binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing; canonical RPA complex; may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC); the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).
Gene Details:
replication protein A1
Molecular Weight:
84 kDa
Protein Details:
Replication protein A 70 kDa DNA-binding subunit
Purity:
Greater than 90% as determined by SDS-PAGE.
Shipping Conditions:
Blue Ice
Source:
E.coli
Storage Conditions:
-20Â[o]C or -80Â[o]C
Supplier:
Aviva Systems Biology
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins