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Trypsin Porcine

Product Sizes
1mg
£186.00
OOPA00226-1MG
About this Product
SKU:
OOPA00226
Extra Details:
Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE); p-toluenesulfonyl- L-arginine methyl ester (TAME); tosyl-L-arginine methyl ester; N-α-benzoyl-L-arginine p-nitroanilide (BAPNA); L-lysyl-p-nitroanilide; and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone); PMSF (phenylmethanesulfonyl fluoride); benzamidine; soybean trypsin inhibitor; and ovomucoid.
Purification:
Purified by standard chromatography techniques.
Shipping Conditions:
Blue Ice
Source:
E.coli
Storage Conditions:
It is recommended to reconstitute the lyophilized Porcine Trypsin in sterile 1mM HCl or 50mM HAC not less than 100 ug/ml; which can then be further diluted to other aqueous solutions. Recombinant Porcine Trypsin although stable at room temp for 1 week; should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Supplier:
Aviva Systems Biology
Type:
Buffers & General Consumables: Others