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Assay Kits & ELISAs

QTRT1 ELISA Kit

Product Sizes
96 Wells
£588.00
OKWB00222-96WELLS
About this Product
SKU:
OKWB00222
Additional Names:
FP3235;guanine insertion enzyme;queuine tRNA-ribosyltransferase 1;queuine tRNA-ribosyltransferase catalytic subunit 1;TGT;TGT; 43-KD subunit;TGT; catalytic subunit;TGUT;tRNA-guanine transglycosylase.
Extra Details:
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp; -Asn; -His and -Tyr); resulting in the hypermodified nucleoside queuosine (7-(((4;5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023; PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA; forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine; allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
Gene Details:
queuine tRNA-ribosyltransferase catalytic subunit 1
Protein Details:
Queuine tRNA-ribosyltransferase catalytic subunit 1
Sensitivity:
0.188 ng/mL
Shipping Conditions:
Blue Ice
Storage Conditions:
Store as indicated in product manual.
Supplier:
Aviva Systems Biology
Type:
Assay Kits: ELISAs