FAM FLICACASPASE-3/7 KIT

Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole; living cells. These kits do not work by using antibodies or as an ELISA. Instead; their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein; FAM) fluorescent probe.

The Caspase FLICA™ Kits are suitable for cells in suspension; adherent cells; thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian; insect and yeast. Different cell types; e.g. HeLa; primary neurons; macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer; fluorescence microscope or a fluorescence plate reader using black microtitre plates.