2, 3-Bisphosphoglycerate ELISA Kit
Product Sizes
96 wells
OKEH02577-96WELLS
About this Product
- SKU:
- OKEH02577
- Additional Names:
- 2, 3-BPG, 2, 3-Bisphosphoglycerate, 2, 3-BPG, 2, 3-Bisphosphoglyceric acid, 2, 3-diphosphoglycerate
- Application:
- ELISA
- Extra Details:
- 2,3-Bisphosphoglyceric acid (2,3-Bisphosphoglycerate or 2,3-BPG, also known as 2,3-diphosphoglycerate or 2,3-DPG) is a three-carbon isomer of the glycolytic intermediate 1,3-bisphosphoglyceric acid (1,3-BPG). 2,3-BPG is present in human red blood cells (RBC; erythrocyte) at approximately 5 mmol/L. It binds with greater affinity to deoxygenated hemoglobin (e.g. when the red cell is near respiring tissue) than it does to oxygenated hemoglobin (e.g., in the lungs) due to spatial changes: 2,3-BPG (whose size is estimated at about 9 angstroms) fits in the deoxygenated hemoglobin configuration (11 angstroms), but not as well in the oxygenated (5 angstroms). It interacts with deoxygenated hemoglobin beta subunits by decreasing their affinity for oxygen, so it allosterically promotes the release of the remaining oxygen molecules bound to the hemoglobin, thus enhancing the ability of RBCs to release oxygen near tissues that need it most. 2,3-BPG is thus an allosteric effector.
- Physical State:
- Cell Culture Supernatant, Other Biological Fluid, Plasma, Serum, Tissue Homogenate
- Range:
- 31.2-2000nmol/mL
- Reactivities:
- Wide/Non-species Specific
- Sensitivity:
- 9.7nmol/mL
- Shipping Conditions:
- Blue Ice
- Specificity:
- Natural and recombinant General 2,3-Bisphosphoglycerate
- Storage Conditions:
- See Manual
- Supplier:
- Aviva Systems Biology
- Type:
- Assay Kits: ELISAs
