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Assay Kits & ELISAs

S100A9 ELISA Kit

Product Sizes
96 Wells
£955.00
OKCD07665-96WELLS
About this Product
SKU:
OKCD07665
Additional Names:
60B8AG;CAGB;calgranulin B;Calgranulin-B;calprotectin L1H subunit;CFAG;CGLB;L1AG;leukocyte L1 complex heavy chain;LIAG;MAC387;MIF;migration inhibitory factor-related protein 14;MRP14;MRP-14;NIF;P14;protein S100-A9;S100 calcium-binding protein A9;S100-A9.
Extra Details:
S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis; adhesion; can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK; PI3K/AKT; and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism; modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane; transferring arachidonic acid; an essential cofactor; to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory; antimicrobial; oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes; promotion of cytokine and chemokine production; and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Has transnitrosylase activity; in oxidatively-modified low-densitity lipoprotein (LDL(ox))-induced S-nitrosylation of GAPDH on 'Cys-247' proposed to transfer the NO moiety from NOS2/iNOS to GAPDH via its own S-nitrosylated Cys-3. The iNOS-S100A8/A9 transnitrosylase complex is proposed to also direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as ANXA5; EZR; MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif.
Gene Details:
S100 calcium binding protein A9
Protein Details:
Protein S100-A9
Sensitivity:
< 5.8pg/mL
Shipping Conditions:
Blue Ice
Storage Conditions:
2Â[o]C to 8Â[o]C|-20Â[o]C
Supplier:
Aviva Systems Biology
Type:
Assay Kits: ELISAs