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Assay Kits & ELISAs

PRKAA1 ELISA Kit

Product Sizes
96 Wells
£814.00
OKCD02822-96WELLS
About this Product
SKU:
OKCD02822
Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-1;ACACA kinase;acetyl-CoA carboxylase kinase;AI194361;AI450832;AL024255;AMP-activated protein kinase; alpha 1 catalytic subunit;AMPK;AMPK subunit alpha-1;AMPKalpha1;C130083N04Rik;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;tau-protein kinase PRKAA1.
Extra Details:
Catalytic subunit of AMP-activated protein kinase (AMPK); an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels; AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein; carbohydrate and lipid biosynthesis; as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes; and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA; ACACB; GYS1; HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes; respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1; PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane; possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2; FOXO3; histone H2B; HDAC5; MEF2C; MLXIPL/ChREBP; EP300; HNF4A; p53/TP53; SREBF1; SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2; leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress; phosphorylates 'Ser-36' of histone H2B (H2BS36ph); leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2; RPTOR and ATG1/ULK1: in response to nutrient limitation; negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation; promotes autophagy by phosphorylating and activating ATG1/ULK1. In response to nutrient limitation; phosphorylates transcription factor FOXO3 promoting FOXO3 mitochontrial import (PubMed:23283301). AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1; leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1; leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure; activated by CAMKK2; leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR; EEF2K; KLC1; NOS3 and SLC12A1.
Gene Details:
protein kinase; AMP-activated; alpha 1 catalytic subunit
Protein Details:
5'-AMP-activated protein kinase catalytic subunit alpha-1
Sensitivity:
0.67 ng/mL
Shipping Conditions:
Blue Ice
Storage Conditions:
2Â[o]C to 8Â[o]C|-20Â[o]C
Supplier:
Aviva Systems Biology
Type:
Assay Kits: ELISAs