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Assay Kits & ELISAs

TOP3A ELISA Kit

Product Sizes
96 Wells
£868.00
OKCD00409-96WELLS
About this Product
SKU:
OKCD00409
Additional Names:
DNA topoisomerase 3-alpha;DNA topoisomerase III alpha;MGRISCE2;PEOB5;TOP3;topo III-alpha;topoisomerase (DNA) III alpha;ZGRF7;zinc finger; GRF-type containing 7.
Extra Details:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme; resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally; in the religation step; the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex; a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
Gene Details:
DNA topoisomerase III alpha
Protein Details:
DNA topoisomerase 3-alpha
Sensitivity:
< 0.053 ng/mL
Shipping Conditions:
Blue Ice
Storage Conditions:
2Â[o]C to 8Â[o]C|-20Â[o]C
Supplier:
Aviva Systems Biology
Type:
Assay Kits: ELISAs